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KMID : 1134820190480010040
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Journal of the Korean Society of Food Science and Nutrition
2019 Volume.48 No. 1 p.40 ~ p.48
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Antioxidant Activity of Suaeda japonica Makino Sprout Extracts
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Kim In-Yong
Ji Seok-Geun
Jeong Yoon-Hwa
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Abstract
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This study examined the antioxidant activities of Suaeda japonica Makino sprouts against oxidatively stressed HepG2 cells. S. japonica Makino sprouts were extracted using a water extract (WE), 50% ethanol extract (50EE), and 100% ethanol extract (100EE). The antioxidant activities in the extracts were measured using a 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay, 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging assay, ferric reducing antioxidant power (FRAP) assay, and oxygen radical absorbance capacity (ORAC) assay. The antioxidant and protective effects for hydrogen peroxide-treated HepG2 cells were evaluated based on the cell viability, intracellular reactive oxygen species (ROS) scavenging activity, and antioxidant enzyme activities, such as superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), and glutathione reductase (GR) in oxidatively stressed HepG2 cells. The highest total flavonoid and phenol contents were 6.68 mg quercetin equivalent/g and 16.67 mg gallic acid equivalent/g, respectively. The DPPH, ABTS, FRAP, and ORAC results also were highest using the 100EE. The DPPH and ABTS using the 100EE were 90.34% and 91.96% at 1,000 μg/mL, respectively. The FRAP using the 100EE was 571.5 μmol FeSO4/g while its ORAC was 1,307.8 μmol Trolox equivalent/g. The results showed that the cell viability was increased by all the extracts. The intracellular ROS scavenging activity was increased 60.4% using 500 μg/mL of the 100EE, respectively. The SOD, CAT, GPx, and GR activities increased to 41.7%, 69.2%, 14.2%, and 31.6%, respectively, using 100EE.
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KEYWORD
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Suaeda japonica Makino sprout, oxidative stress, antioxidant activity, HepG2 cell
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